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phenol red free opti mem tm i reduced serum medium  (Thermo Fisher)


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    Thermo Fisher phenol red free opti mem tm i reduced serum medium
    Phenol Red Free Opti Mem Tm I Reduced Serum Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/opti-mem+i+reduced+serum+medium/Phenol+Red/bio_rxiv__64898__2026__04__10__717554-212-58-66
    Average 99 stars, based on 1 article reviews
    phenol red free opti mem tm i reduced serum medium - by Bioz Stars, 2026-10
    99/100 stars

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    Virus:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    Recombinant:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    High Throughput Screening Assay:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    RNA Sequencing:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    Plasmid Preparation:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    Expressing:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    Cell Culture:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.

    Transfection:

    Article Title: Multimodal CRISPR screens uncover DDX39B as a global repressor of A-to-I RNA editing.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-ADAR1 Cell Signaling Technology Cat#81284S; RRID:AB_3068597 Mouse monoclonal anti-DYKDDDDK (HRP) Absin Bioscience Cat#abs830014; RRID:AB_3683650 Rabbit monoclonal anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Rabbit monoclonal anti-UAP56 (DDX39B) Abcam Cat#ab181061; RRID:AB_3683651 Mouse monoclonal anti-ADAR2 Santa Cruz Biotechnology Cat#sc-73409; RRID:AB_2289194 Mouse monoclonal anti-SC35 Abcam Cat#ab11826; RRID:AB_298608 Mouse monoclonal anti-dsRNA, clone rJ2 Millipore Cat#MABE1134; RRID:AB_2819101 Mouse monoclonal anti-dsRNA SCICONS Cat#10010200; RRID:AB_2651015 Mouse monoclonal anti-HDAg Kerafast Cat#EHD001; RRID:AB_3696045 Bacterial and virus strains HDV infectious clone ZZ173 This paper N/A Stbl3 chemically competent E. coli ALPALIFEBIO Cat#KTSMCC1200 Chemicals, peptides, and recombinant proteins DMEM Thermo Fisher Scientific Cat#C11995500BT DMEM/F12 Thermo Fisher Scientific Cat#C11330500BT Opti-MEM I Reduced Serum Medium GIBCO Cat#31985070 Polyethylenimine Linear (PEI) MW40000 Yeasen Cat#40816ES03 Lipofectamine RNAiMax Reagent Invitrogen Cat#13778150 Puromycin solution Yeasen Cat#60209ES50 RNase A Yeasen Cat#10406ES03 DAPI Beyotime Cat#C1006 Critical commercial assays MolPure® Cell RNA Kit Yeasen Cat#19231ES50 Chip A Single Cell Kit v2.1 MobiDrop Cat#S050100301 High Throughput Single-Cell 3′ Transcriptome Kit v2.1 MobiDrop Cat# S050200301 3′ Dual Index Kit MobiDrop Cat# S050300301 Easy II Protein Quantitative Kit TransGen Cat# DQ111 Deposited data Raw bulk RNA-seq and scRNA-seq data This paper GSA-Human: HRA011402 Processed data This paper Zenodo: https://doi.org/10.5281/zenodo.

    Article Title: Protocol for real-time glycolytic monitoring in mammalian cells using confocal microscopy and HYlight, a biosensor for fructose 1,6-bisphosphate
    Article Snippet: Warm up Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific), cell culture media, and PBS to 37°C. c. Prepare per single Ibidi well, using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific): Tube A: 1 μg of HYlight-pCS2+ plasmid, 0.5 μL of P3000, and 12.5 μL of Opti-MEM.

    Article Title: A Novel Modulator of Resistance for Oxaliplatin-Based Therapy for Colorectal Cancer: The ESCRT Family Member VPS4A
    Article Snippet: The cells were all maintained in RPMI 1640 culture medium supplemented with 10% ( v / v ) fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, 2 mmol/L of L-glutamine (all from Merck, Gillingham, UK), apart from HIEC-6, which was grown in Opti-Mem I reduced serum Medium supplemented with 20 mM HEPES, 10 mM GlutaMax, 10 ng/mL Epidermal growth factor (EGF), and 4% fetal bovine serum (FBS) (all from Thermofisher Scientific, Loughborough, UK).

    Article Title: Chromatin interaction-based annotation of regulatory elements reveals dynamic promoter-enhancer interactions in lymphocyte development
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , Cat#31985-054.

    Article Title: Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids
    Article Snippet: At 60% confluence, 7.5 μg psPAX2 packaging plasmid (Addgene 12260), 750 ng pMD2.G envelope plasmid (Addgene 12259), 7.5 μg lentiviral expression plasmid (Supplementary Note ), and 15 μL PLUS reagent (Lipofectamine LTX kit) were mixed in 750 μL Opti-MEM I reduced serum medium (Thermo Fisher Scientific).

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Opti-MEM I Reduced Serum Medium , Thermo Fisher Scientific , cat. no. 31985062.

    Article Title: Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and shape their functional biology
    Article Snippet: Plasmids were diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific) along with P3000 at a ratio of 2 μl of P3000 per microgram of DNA.

    Article Title: CALR Type 1-Like Mutations Increase Endoplasmic Reticulum Free Ca 2+ and Induce ERK1/2 Activation Independent of Thrombopoietin Receptor Activation.
    Article Snippet: Secretome samples were prepared by plating cells in Opti‐MEM I Reduced Serum Medium (ThermoFisher 11058021) in a 6‐well plate.



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